anti-tdTomato
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Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.
- Purification: Epitope affinity purified
- Immunogen: Purified recombinant peptide produced in E. coli.
- Specificity test:In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
- Buffer: PBS, 20% glycerol and 0.05% sodium azide
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- Concentration: 3 mg/mL
- Conjugation: Unconjugated
- Clonality: Polyclonal
- Istotype: IgGHost Species: goat
- Confirmed Reactive Species: tdTomato, mCherry, RFP
- Target Name: tdTomato
- Applications: WB, IF, IHC-P, IHC-F, IEM
- Dilutions by application: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
For research use only.
