Ubiquitin Detection Kit

The covalent attachment of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events including cell cycle, differentiation, immune responses, DNA repair, chromatin structure, transcription, signal transduction, endocytosis, apoptosis and degradation by the proteasome, autophagy and lysosome systems. As such ubiquitin signalling and the processes it mediates are essential for the normal functioning of cells and its dysfunction has been implicated in wide range of diseases including cancer, neurodegeneration, cardiovascular and metabolic disorders (1,2). The type of ubiquitin modification, (monoubiquitin, multiubiquitin, polyubiquitin), substrate protein lysine residue(s) modified and, in the case of polyubiquitination, the chain length and lysine linkage type control the function and fate of ubiquitinated proteins. In addition all ubiquitin mediated pathways also utilise speci?c ubiquitin receptors to facilitate their regulation (3,4). Ubiquitination is achieved through three enzymatic steps. In an ATP-dependent process, the ubiquitin E1 activating enzyme catalyses the formation of a reactive thioester bond with ubiquitin, followed by its subsequent transfer to the active site cysteine of a ubiquitin E2 carrier protein. The selectivity of the ubiquitin cascade for a particular substrate protein relies on the interaction between the E2 conjugating enzyme (of which a cell contains relatively few) and an ubiquitin E3 ligase , of which over 600 have been identified to date. The specific E2-E3 pair required for ubiquitination of a particular substrate protein in vivo may also control the type, point and length / linkage (polyubiquitin) of the ubiquitin modification (5).

• Assay type: Binding Matrix/Quantitative WB (Western Blot)
• Species reactivity: Species Independent
• Sample types: Cell Lysates, Tissue
• Incubation t 1 hour
• Number of samples per kit: 20 samples

The kit is transported on ice packs (+4ºC). Upon receipt store at 4ºC. Non-hazardous item according to both ADR and UN regulations.

For research use only. Not to be used for human or animal treatment or diagnostics.