TIGIT Recombinant Protein
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T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
- Tested Applications: E, WB
- Applications: This recombinant protein can be used for E, WB. For research use only.
- Predicted Molecular Weight: 40 kDa
- Physical state: Lyophilized
- Buffer: 50 mM Tris, 100 mM Glycine, pH7.5
- Concentration: N/A
- NCBI official symbol: TIGIT
- Accession #: G7NXM4
- Protein GI: N/A
- NCBI gene ID#: N/A
- NCBI official full name: N/A
- NCBI organism: Macaca fascicularis
- Peptide sequence: Met 89 - Ile 209
- SWISSPROT #: G7NXM4
- Background Reference 1: Yu X., et al., 2009, Nat. Immunol. 10:48-57.
- Background Reference 2: Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
- Background Reference 3: Lozano E., et al., 2012, J. Immunol. 188:3869-3875.
- Background Reference 4: N/A
- Background Reference 5: N/A
- Source: HEK293 cells
- Species: Cynomolgus, Rhesus macaque
- By Source: Human Cells
- By Species: Other
- Fusion tag: Fc Tag
- Sequence: Met 89 - Ile 209
- Biology activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD155, His Tag at 10 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Fc Tag with a linear range of 2.22-20 ug/mL.
- Purity: >95% as determined by SDS-PAGE.
- Endotoxin level is less than 1.0 EU per ug by the LAL method.
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
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