Serpin A1 Recombinant Protein
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Serpin A1 is also known as Alpha-1-antitrypsin (A1AT), serum trypsin inhibitor, alpha-1 proteinase inhibitor (A1PI), AAT, which belongs to the serpin family. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Serpin A1 / A1AT is inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of SerpinA1 inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Serpin A1 / A1AT protects tissues from enzymes of inflammatory cells, especially neutrophil elastase. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD).
- Tested Applications: WB
- Applications: This recombinant protein can be used for WB. For research use only.
- Predicted Molecular Weight: 45.1 kDa
- Physical state: Lyophilized
- Buffer: PBS, pH7.4
- Concentration: N/A
- NCBI official symbol: SERPINA1
- Accession #: AAH11991
- Protein GI: N/A
- NCBI gene ID#: 5265
- NCBI official full name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
- NCBI organism: Homo sapiens
- Peptide sequence: N/A
- SWISSPROT #: P01009
- Background Reference 1: Tanaka N., et al., 1991, Jpn. J. Cancer Res. 82:693-700.
- Background Reference 2: Niemann M.A., et al., 1992, Matrix 12:233-241.
- Background Reference 3: Gettins PG., 2002, Chem Rev 102 (12): 4751–804.
- Background Reference 4: N/A
- Background Reference 5: N/A
- Source: HEK293 cells
- Species: Human
- By Source: Human Cells
- By Species: Human
- Fusion tag: His Tag
- Sequence: Glu 25 - Lys 418
- Biology activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in 100µl reaction mixture containing 1. 25 ng trypsin, 10µM substrate, 50 mM Tris, 10 mM CaCl2, 0. 15 M NaCl , pH 7. 5. The IC50 value is approximately <4. 5 nM.
- Purity: >95% as determined by SDS-PAGE.
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
