PAK4 Antibody
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PAK4 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- Research area: Signal Transduction, Neuroscience, Cancer
- Host species: Rabbit
- Species reactivity: Human
- Homology: N/A
- Immunogen: PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4.
- The immunogen is located within amino acids 210 - 260 of PAK4.
- Tested Applications: E, WB, IHC-P, IF
- Applications: PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 μ
- g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ
- g/mL. For immunofluorescence start at 20 μ
- g/mL.
- Antibody validated: Western Blot in human samples
- Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- Specificity: PAK4 antibody is predicted to not cross-react with other PAK family proteins.
- Positive control 1: Cat. No. 1221 - SW480 Cell Lysate
- Positive control 2: Cat. No. 1320 - Human Colon Tissue Lysate
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: Predicted: 65 kDa
- Observed: 70 kDa
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: PAK4 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: PAK4 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: PAK4
- Accession #: NP_005875
- Protein GI #: 5031975
- NCBI gene ID#: 10298
- NCBI official full name: p21 protein (Cdc42/Rac)-activated kinase 4
- NCBI organism: Homo sapiens
- SWISSPROT #: O96013
- PAK4 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Optimal dilutions for each application to be determined by the researcher.
