Human Normal Tissue Blot VI
€ Preis anfragen
Ask for price
The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
- Tested Applications: WB
- Applications: For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
- Species: Human
- Samples loaded: Lanes: 1, protein markers
- 2, left cerebellum
- 3, right cerebellum
- 4, frontal lobe
- 5, occipital lobe
- 6, parietal lobe
- 7, whole eye
- 8, spinal cord
- 9, temporal lobe
- and 10, thalamus.
- Quantity loaded per lane: 15 µg
- Gel percentage: Gradient 4-20%
Store at 4°C. The blot can be reused after stripping.
- Lysate lane order may vary
- please consult accompanying shipment paperwork.
