Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

On January 11, 2020, Chinese health authorities preliminarily identified more than 40 human infections with a novel coronavirus in an outbreak of pneumonia under investigation in Wuhan City, Hubei Province, China.The Chinese authorities identified a new type of coronavirus (novel coronavirus, named as 2019-nCoV), which was isolated on 7 January 2020.Coronaviruses are a large family of viruses, some causing illness in human and others circulating among animals such as camels, cats and bats. 2019-nCoV is a novel coronavirus. The primer andprobe design for this kit is based on the newly released strain (2019-nCoV) (GeneBank accession: MN908947) and covers 6 2019-nCoV strains sequences (EPI_ISL_402119, EPI_ISL_402120, EPI_ISL_402121, EPI_ISL_402122, EPI_ISL_402123, EPI_ISL_402124 included).Thekit contains a specific ready-to-use system for the detection of NovelCoronavirus (2019-nCoV) by Reversetranscription Polymerase Chain Reaction(RT-PCR) in the real-time PCR system. The master contains a Super Mix for the specific amplification ofvirusRNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the virusRNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of polymerase chain reaction (PCR). Fluorescence is emitted and measured by the real time systems ́ optical unit during PCR. The detection of amplified virusDNA fragment is performed in fluorimeterchannelFAMand channel HEX/VIC/JOEwith the fluorescent quencher BHQ1. In addition, the kit contains a systemto identify possible PCR inhibition by measuring the CalRed 610/ROX/TEXAS RED fluorescence of the internal control (IC).

• Shipping: dry ice
• All reagents should be stored at -20°C. Storage at +4°C is not recommended.