ELISA kit for NA/NE (Noradrenaline/Norepinephrine)
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A competitive ELISA kit for quantitative measurement of NA/NE (Noradrenaline/Norepinephrine) in samples from Serum, Plasma, Cell supernatant
Gentaur's NA/NE ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NA/NE in the samples is then determined by comparing the OD of the samples to the standard curve.
- Species reactivity: Universal
- Antigen: NA/NE
- Assay type: Competitive ELISA
- Sensitivity: 0.19ng/mL
- Detections range: from: 0.31 to: 20ng/mL
- Validated sample types: Serum, Plasma, Cell supernatant
The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.
This Universal NA/NE ELISA kit is intended for research use only. Do not use for human or animal diagnostics.