EasyScript Plus Reverse Transcriptase (PCR)

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Information

Catalog numberG177
NameEasyScript Plus Reverse Transcriptase (PCR)
Size25 x 20 ul reactions
Price74.00EUR
SKU01013734218
SupplierABM PCR reagents
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Details

Product categoryPCR, RT-PCR & qPCR
ApplicationPCR. For others please contact us
LiteratureRefer to NCBI PubMed for current references.
Technical datasheetPlease contact us to receive a datasheet or any other useful technical documentation available.
GroupPCR, polymerase chain reaction
AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
DescriptionReverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goupreverse transcription