Flag and His tagging of cytoplasmic and nuclear proteins in baculovirus system

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Catalog numberB9-50
NameFlag and His tagging of cytoplasmic and nuclear proteins in baculovirus system
Size50 μL
Price349.00EUR
SKU01014298545
SupplierVector
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Details

DescriptionFlag and His tagging of cytoplasmic and nuclear proteins in baculovirus system (pVL-FH)
Produced inSpodoptera frugiperda, Trichoplusia ni
ApplicationProtein expression
Gene numberPlease refer to Genebank
DeliveryShipped under blue ice
StorageStore at plus 4°C
Technical filePlease inquire the latest datasheet, protocol, certificate of analysis or SDS
PropertiesAn anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
ConjugationFlag,histidine