anti-6xHIS Host: Rabbit FITC Conjugated

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Information

Catalog numberRHIS-45F-Z
Nameanti-6xHIS Host: Rabbit FITC Conjugated
Size0.1 mg
Price191.00EUR
SKU01011987509
Suppliericl
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ConjugatedThe polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Most polyclonal antibodies pabs are raised in rabbits as rabbit polyclonal antibodies.
PropertiesThis icl Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. icl FITC is highly sensitive to pH extremes.
ConjugationAnti-FITC Antibody
AboutRabbits are used for polyclonal antibody production by icl. Rabbit antibodies are very stable and can be stored for several days at room temperature. icl adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin nameOryctolagus cuniculus