anti-6xHIS Host: Rabbit Biotin Conjugated

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Catalog numberRHIS-45B-Z
Nameanti-6xHIS Host: Rabbit Biotin Conjugated
Size0.1 mg
Price191.00EUR
SKU01011987508
Suppliericl
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ConjugatedThe polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Most polyclonal antibodies pabs are raised in rabbits as rabbit polyclonal antibodies.
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
ConjugationBiotinylated
AboutRabbits are used for polyclonal antibody production by icl. Rabbit antibodies are very stable and can be stored for several days at room temperature. icl adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin nameOryctolagus cuniculus