Siglec-8 Recombinant Protein
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Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2). At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.
- Tested Applications: WB
- Applications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.3 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Predicted Molecular Weight: 64.3 kDa
- Physical state: Lyophilized
- Buffer: PBS, pH7.4
- Concentration: N/A
- NCBI official symbol: SIGLEC8
- Accession #: NP_055257.2
- Protein GI: N/A
- NCBI gene ID#: 27181
- NCBI official full name: sialicacidbindingIglikelectin8
- NCBI organism: Homo sapiens
- Peptide sequence: N/A
- SWISSPROT #: Q9NYZ4
- Background Reference 1: Floyd H, et al. 2000. J Biol Chem. 275(2):861-6.
- Background Reference 2: Foussias G, et al. 2000. Biochem Biophys Res Commun. 278(3):775-81.
- Background Reference 3: Kikly KK, et al. 2000. J Allergy Clin Immunol. 105(6 Pt 1):1093-100.
- Background Reference 4: N/A
- Background Reference 5: N/A
- Source: HEK293 cells
- Species: Human
- By Source: Human Cells
- By Species: Human
- Fusion tag: Fc Tag
- Sequence: Met 17 - Ala 363
- Biology activity: N/A
- Purity: >95% as determined by SDS-PAGE.
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
