Interleukin 1b Recombinant Protein
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Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta.IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
- Tested Applications: WB
- Applications: This recombinant protein can be used for WB. For research use only.
- Predicted Molecular Weight: 17.5 kDa
- Physical state: Lyophilized
- Buffer: PBS, pH7.4
- Concentration: N/A
- NCBI official symbol: IL1B
- Accession #: NP_000567
- Protein GI: N/A
- NCBI gene ID#: 3553
- NCBI official full name: interleukin 1, beta
- NCBI organism: Homo sapiens
- Peptide sequence: N/A
- SWISSPROT #: P01584
- Background Reference 1: Auron PE, Webb AC. Proc Natl Acad Sci U S A 81 (24).
- Background Reference 2: March CJ, Mosley B, Larsen A. Nature 315 (6021): 641–7.
- Background Reference 3: Clark BD, Collins KL, Gandy MS, Webb AC, Auron PE. Nucleic Acids Res 14 (20): 7897–1914.
- Background Reference 4: Martinon, F. and J. Tschopp, 2007, Cell Death Differ. 14:10.
- Background Reference 5: N/A
- Source: E. coli
- Species: Human
- By Source: E. Coli
- By Species: Human
- Fusion tag: Tag free
- Sequence: Ala 117 - Ser 269
- Biology activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10. G4. 1 cells. The ED50 < 2 pg/mL, corresponding to a specific activity of >500,000,000 Unit/mg.
- Purity: >97% as determined by SDS-PAGE.
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
