Spoligotyping Kit with Primers and Controls
€ Preis anfragen
A very simple, inexpensive and effective tool for Tuberculosis/ Mycobacterium research. Spoligotyping is a PCR-based Method to Simultaneously Detect and Type Mycobacterium Tuberculosis Complex Bacteria. Spoligotyping, which uses RLB (Reversed Line Blotting) offers an alternative for typical Southern blotting when rapid results are required. The method is particularly useful to simultaneously detect and type M. tuberculosis complex bacteria in clinical samples (suspected nosocomial infections, outbreaks in prisons, etc.). The level of differentiation by spoligotyping is less compared to IS6110 fingerprinting for strains having five or more IS6110 copies, but higher for strains with less than five copies. Thus, Spoligotyping is a preferred method to type M. bovis strains, which usually contain only one or two IS6110 copies. Note, that Mycobacterium bovis can be recognized by the absence of reactivity with spacers 39-43.
The kit consists of four vials, one membrane and manual.
- Two vials containing the positive controls:
- positive control 1 ( M. tuberculosis strain H37Rv )
- positive control 2 ( M. bovis BCG P3 )
- Two vials containing the primers for PCR amplification:
- primer Dra ( biotinylated )
- primer Drb
- One Spoligo-membrane. The membrane is shipped in 20 mM EDTA.
- Membrane to be stored at + 4°
- C, for optimal storage condition.
- Primer DRa is biotinylated, should be stored at +4°
- C.
- Primer DRb to be stored in small aliquots at -20°
- C.
- Controls 1 &
- 2, are to be stored at -20°
- C.
