ELISA kit for Human VAMP2 (Vesicle Associated Membrane Protein 2)
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A sandwich ELISA kit for detection of Vesicle Associated Membrane Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicle Associated Membrane Protein 2 (VAMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vesicle Associated Membrane Protein 2 (VAMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vesicle Associated Membrane Protein 2 (VAMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
- 10nm. The concentration of Vesicle Associated Membrane Protein 2 (VAMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Species reactivity: Human
- Sensitivity: 0.055ng/mL
- Detection range: 0.156-10ng/mL
- Standard solution concentration: 10ng/mL
- ELISA type: Sandwich
- Assay length: 3h
The Human VAMP2 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
This kit is intended for research use only. Do not use for diagnostics purposes.
