Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12)
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Western blot, 0.1-0.5μg/ml;_x000D_Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml;_x000D_Immunocytochemistry/Immunofluorescence, 2μg/ml;_x000D_Flow Cytometry, 1-3μg/1x106 cells
Human,Mouse,Rat
- Clonality: Monoclonal
- Ig type: Mouse IgG2a
- Form: Lyophilized
- Specificity: No cross reactivity with other proteins.
- Reactivity: Reacts with: human
- Application: WB,IHC-P,ICC/IF,FCM
- Notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Immunogen: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively.
- Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
- Purification: Immunogen affinity purified.
- ReconstitutionAdd 0.2ml of distilled water will yield a concentration of 500μg/ml.
- Gene Name: LGALS3
- Protein Name: Galectin-3
- Gene Full Name: lectin, galactoside-binding, soluble, 3
- Uniprot ID: P17931
- Entrez GeneID: 3958
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
