Cas9 Nuclease Protein (High Concentration)

Der Lieferant: Gentaur

0
( 0 Überprüfung )

€ Preis anfragen

424.60 EUR

Katalognummer: 10021-K108

Größe: 40 µg (250pmol) Volume: 25µL

Cas9 Nuclease Protein (High Concentration)
Cas9 Nuclease Protein (High Concentration)

Catalog number: / Size: / Price: EUR (Excluded VAT)

Inquiry cart
1
User Email: | Edit
2
Request Info
Cas9 Nuclease Protein (High Concentration)
Cas9 Nuclease Protein (High Concentration)

Catalog number: / Size: / Price: EUR (Excluding VAT)

3
Inquiry cart
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.
For research use only.

0 Bewertungen für dieses Produkt

Bewertung hinzufügen

Deine Email-Adresse wird nicht veröffentlicht. Erforderliche Felder sind markiert *

1 2 3 4 5

Main Menu