ELISA kit for Free Thyroxine (fT4)
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Quantitative competitive ELISA for measuring Free Thyroxine (fT4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
- Over 99% of Triiodothyronine (T4) circulates in blood is bound to carrier proteins
- thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T4 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.
- Reactivity: Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine
- Sample types: serum, whole blood, plasma and other biological fluids
- Detection range: 4 pmol/L-64 pmol/L
- Sensitivity: 4.0 pmol/LQuantitative Competitive ELISA
- Method of detection: Colorimetric
All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.
